FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630896
Sequences flagged as poor quality0
Sequence length44-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT59050.023.0931191
TATTAGA66400.022.5187172
CGACCCG140450.022.4036245
GTATTAG84900.022.0850071
TAGCCCC67250.020.756215
GTGTTAT145800.019.9073281
TGTTATG140000.019.1299152
TGTGCGT115200.018.9761418
CGGTTCG63100.018.8218679
ACCCGCG142150.018.8070187
GTTATGC145100.018.0659473
CTATAGT27350.017.9926174
CGGGACG108300.017.8695325
TCTATAG32450.017.497863
TATAGTG31950.017.4754945
CGGTGGT520200.017.3991694-95
GCGTAGC80000.017.2118552
GTATCAT72950.017.0287194-95
TAGATAG25950.016.9575825
TCGGTGG610800.016.61478894-95
Produced by FastQC (version 0.11.5)