FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12491576
Sequences flagged as poor quality0
Sequence length88-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA815720.6530160805970359No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT650320.5206068473665773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC31850.025.541725
CTATAGT13350.023.3826524
GCGTAGC35450.023.0823542
TGTGCGT59600.023.014898
GCCGTCT300700.022.77362394-95
CGCGTGT33150.021.5449451
GTATTAG58750.020.6102891
TCTATAG15100.019.7330723
AATACGG29000.019.2448775
GTGTGCG74850.018.7039267
TGCCGTC390650.018.6053794-95
CGTAGCC48150.018.4668433
TACACTA13000.017.8272295
TAATACT49650.017.432634
TACGGAG30200.017.2273547
TAGGGTC48100.016.9127584
CGACCCG55450.016.8886435
GTGCGTT29750.016.85194432-33
TATAGTG17300.016.6768825
CCGCGCG58850.016.5569929
Produced by FastQC (version 0.11.5)