FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12491576
Sequences flagged as poor quality0
Sequence length43-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA763800.6114520697788655No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT618290.49496556719504403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA18650.022.78258594-95
GTATTAG60300.020.645161
TAGCCCC29000.020.3904845
CTATAGT14450.018.9878444
TGTGCGT53500.018.3918368
TCGGTGG253950.018.23200294-95
CGCGTGT31900.017.8061661
AATACGG26850.017.7947795
CGGTGGT194200.017.4551194-95
CGACCCG58700.017.407295
GCGTAGC33250.017.35752
CTACACT42700.017.0611534
TATTAGA63250.016.7535362
TAATACT49800.016.718564
TAGGGTC51500.016.7178214
TAGATAG19950.016.598575
TATAGTG19400.016.0937675
TACGGAG29350.015.9566837
TACACTA13850.015.7117155
CATAGGG65650.015.4925442
Produced by FastQC (version 0.11.5)