FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750175
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA715500.48507899058824727No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT575590.3902258786760157No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT181720.12319853832242669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT46400.036.9250531
TGTGCGT97350.026.0110958
TAGCCCC38400.024.8963665
GCCGTCT472700.024.61754494-95
GTATTAG29300.024.230041
GCGTAGC40150.024.0476252
TAGATAG16550.023.4493965
GTGTGCG111450.022.7190157
AATTTAA84700.021.5690732
GTGCGTC124550.021.0532979
ATTTAAG87450.020.9443513
CGGGACG49150.020.3177095
GCTTGAA118450.020.25643794-95
TGCCGTC602400.020.09500394-95
CGTGTGG126900.019.5061071
CGTAGCC65750.019.4349673
AAGCATA94050.019.4242527
TAAGCAT94250.019.3328196
TATTAGG12650.018.7071232
TGGTGTG159150.018.4374495
Produced by FastQC (version 0.11.5)