FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750175
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA682260.4625436647361811No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT534030.36204994178035177No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT210190.14250000423723785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151120.10245302174380982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT35100.031.0291611
TAGATAG15450.025.1159135
TGTGCGT82800.024.3468558
TAGCCCC35650.022.0349565
GTGTGCG93650.022.031417
GCGTAGC38100.020.866492
CGGTGGT290050.020.08041294-95
AATTTAA78650.019.6148032
GTATTAG28200.019.478621
TAGGCCC23800.019.286724
CGGGACG48000.019.2245865
ATTTAAG80700.019.175113
GTGCGTC115350.018.9944639
TTAGAAC26900.018.6473543
AAGCATA82900.018.6091587
TAAGCAT84850.018.34886
TCGGTGG392850.017.98377294-95
GTATCAT33350.017.87944894-95
TAAGGTA27200.017.7457715
ATATTAG104600.017.655591
Produced by FastQC (version 0.11.5)