FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41004198
Sequences flagged as poor quality0
Sequence length86-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGT616880.15044313267631768No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA548730.13382288320820224No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA468740.11431512451481188No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT455150.11100082971992284No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT410400.100087313011219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT145550.049.8482481
CGTGTGG377250.032.656141
GCGTGTG227750.032.230932
TGTGCGT397450.031.4854228
GTGTGGT401850.030.3625582
GTGTGCG436900.028.9982647
GTGCGTC456950.027.3757769
CGGGACG184400.026.640455
GTGGTGT502450.025.2339344
TGGTGTG531650.024.8272045
GCCGTCT2549800.023.3144194-95
CGGTTCG50150.022.461779
GGTGTGC591550.022.0892316
CTTATGG64650.021.8880863
GAATTTA95500.021.0622861
GATAGTT43350.020.9628379
ATATTAG133250.020.5651231
TGCCGTC2998700.020.18041494-95
TGTGGTG625950.020.1040633
GTATTAG180750.019.9262831
Produced by FastQC (version 0.11.5)