FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41004198
Sequences flagged as poor quality0
Sequence length35-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA528040.12877705838802164No Hit
ACCCCACACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACC479710.1169904603426215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG465710.11357617578570858No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA443410.10813770824148299No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT417240.1017554348947393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT110900.035.8574941
CGGTTCG134750.029.6660064
TGTGCGT258250.027.6754288
CGGGACG174600.025.9876295
GTGTGCG300700.024.1622497
GTGCGTC319400.022.4362099
GATAGTT38500.022.267089
GTATCAT47100.022.01557594-95
GGTTCGT183000.021.7148255
CTTATGG71550.021.4477043
GCGTGTG185600.021.385192
GAATTTA85000.020.7988551
TACCGGT29150.020.7977737
ATATTAG114300.020.5261961
GTATTAG182150.020.5043741
CCTACCC196950.020.489393
GCCCTAA53750.020.3696231
TACCCCC144350.020.2448145
TATTAGA163300.019.9258822
GCCCTAC182750.019.6589971
Produced by FastQC (version 0.11.5)