FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15031168
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA625850.41636817577981966No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA515380.34287421975457927No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG303430.20186721351261594No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC279010.18562097103831185No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG212690.1414993166199726No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC192370.12798073975355742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169340.11265924244875715No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA159900.10637895870766663No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG152090.10118308836678559No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA151110.1005311097580707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAT212000.030.0857491
TGTTATG214300.029.5009422
TATTAGA104300.028.1293032
GTATTAG124200.027.1448481
GTTATGC236950.026.4213333
TTATGCC247750.025.1549644
CATAGGG98600.023.9003372
TATGCCC261900.023.850095
TTAGAAC37100.023.469053
TAGGGTC98300.023.4437484
CCATAGG99700.023.0343321
ATGCCCG273150.022.7465296
TGCCCGC286400.021.7602737
ATTAGAG151750.020.4874673
TACCCCG67450.020.4156235
ATAGGGT117850.020.0766873
CGCGTGT21600.019.9487951
TGTGCGT51000.019.763378
TAGAGGC164450.019.1067055
CGGGACG35000.018.9282995
Produced by FastQC (version 0.11.5)