FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18303790
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT340610.18608714370084012No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT240600.131448186413852No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACA229210.12522543145436No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA222740.12169064439659764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201040.10983517621213969No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCA197170.10772085999675478No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG194660.10634955929892116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAC89850.026.9029143
ACTCTCG92800.025.8559551
GTATTAG57750.024.994731
TCGACTG103300.023.6290325
CTCGACT107550.022.6513124
TAAGGCG38000.022.1581235
CTCTCGA110700.022.0070632
GTATCAT61250.021.01219294-95
GGTTAAG39500.020.35892
TATTAGA50750.020.3199622
CGGTGGT398950.018.87944494-95
CGACTGC134000.018.5332266
TAGATAG18950.018.4722185
GGGTTAA45350.018.4712621
GGACGGT154250.018.338876
TCGGTGG491200.018.1414694-95
TAGGGTC96000.016.8524
ACTGCCG149650.016.8479488
TATAGAC75750.016.7983323
CGTATCA44100.016.64836394-95
Produced by FastQC (version 0.11.5)