FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30314944
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT762600.022.42578794-95
TAGCCCC70350.020.5780035
CGCGTGT83500.019.5475731
GTGTTAT153650.019.4292931
TAGATAG25650.019.181915
GTATTAG97000.018.6804471
TGTTATG156150.018.3000532
GTTATGC152200.018.246073
TGCCGTC957850.018.19395694-95
CGACCCG123250.018.0024575
TATTAGA68950.017.6342182
TGTGCGT135300.017.3443078
CCGTCTT633300.016.32170994-95
TTATGCC177850.016.0667844
TTAGAAC83150.015.8171573
GTATTGG143550.015.4571661
TAGGGTC95050.014.7328064
TAAGGTA60050.014.6536495
ATGCCGT991600.014.39978492-93
CTACACT102050.014.3712514
Produced by FastQC (version 0.11.5)