FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30314944
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA48950.024.99031894-95
TATTAGA67900.022.1594982
GTATTAG99350.022.1541981
TAGATAG25950.020.7858225
GTGTTAT153700.019.2476231
CGACCCG131200.017.959545
GTTATGC152200.017.782023
TGTTATG156200.017.7508492
TCGGTGG601550.017.1569294-95
TAGCCCC66750.017.0830545
CGGTGGT481550.016.63017394-95
GTATTGG142550.016.3700731
TGTGCGT120950.016.2737058
GTATCAT73650.015.93115794-95
TTATGCC174800.015.7807214
TTAGAAC89750.015.4993183
CGCGTGT74850.015.4301081
TAGGGTC94800.015.0729524
GAGTAGG113500.014.7631261
TAGAACT130350.014.6645944
Produced by FastQC (version 0.11.5)