FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005406488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5306014
Sequences flagged as poor quality0
Sequence length89-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGAC225220.4244617522682752No Hit
CTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGG217620.41013838259755814No Hit
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC174170.3282501704669456No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA100560.18952079659043491No Hit
GCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT92790.1748770357560308No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT90540.17063656447193692No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG75080.141499815115452No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG73960.1393890027429253No Hit
GGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGAC72190.13605316533277145No Hit
GGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCT71950.13560084839580144No Hit
CGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCC71200.13418735796777015No Hit
GAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCG68160.12845801009948335No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA54560.10282671700451601No Hit
TCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGA53880.10154515234976763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)