FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21992268
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC56400.027.7607675
GCGTAGC64150.024.1863672
TAGATAG28450.023.60965
GCCGTCT411800.023.09316694-95
GTATTAG90900.020.4513571
TGTGCGT96000.020.1540938
CGTAGCC85500.019.3634833
CGCGTGT58900.018.3913521
TTAGAAC65750.018.2734093
TGCCGTC563100.018.21391594-95
TATTAGA64950.018.2076662
GTGTGCG121000.016.4580847
GGCGTAG112350.016.1257211
CCGTCTT381700.016.01847394-95
GTGCGTT58800.015.92830132-33
GTGCGTC134750.015.6925639
AGGACGC99550.015.348879
CTACACT92200.015.3398774
GTATTGG95650.015.3309881
TAGAACT92900.015.1224564
Produced by FastQC (version 0.11.5)