FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21992268
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238440.10841992285652394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC53850.025.0465565
GCGTAGC60200.022.2474772
TGTGCGT91800.020.8785928
TAGATAG26950.020.5454655
GTATTAG89600.019.554081
TATTAGA59650.019.4377732
CGCGTGT44500.017.9833411
CGTAGCC84800.017.132923
TTAGAAC66650.016.7571723
GTGTGCG114450.016.6639567
TAGGGTC78600.016.2566134
TAGAACT87400.015.6485924
GTATTGG92250.015.6045371
GGCGTAG105900.015.1134771
GTGCGTT54650.015.111032532-33
ATAGGGT87450.015.098483
TAATACT58700.015.0762174
GTGCGTC132450.014.9352749
GTACTAA21150.014.7766931
TAAGGTA52250.014.5823815
Produced by FastQC (version 0.11.5)