FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28175736
Sequences flagged as poor quality0
Sequence length86-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942190.3343976533567748No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT896930.318334186549732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG27850.022.9273535
TGTGCGT111000.021.0513958
GTATTAG83450.020.8584961
GCCGTCT503350.020.57078494-95
CGACCCG113650.020.22615
GGTAATA77850.020.1715562
GTAATAA83700.017.9134453
CGCGTGT56100.017.7058981
TAATAAT87150.017.4213984
TGCCGTC651700.016.652694-95
CCGTACG44050.016.321412
GTGTGCG145400.016.2975277
GGGTAAT108050.016.2409211
ATTTAAG121100.016.2087423
TTAGAAC65400.015.9830573
TAGGCCC48600.015.9607364
CCGCGCG113350.015.7327439
TATTAGA51650.015.6597832
GTGCGTC154750.015.6503469
AATTTAA127800.015.4335272
Produced by FastQC (version 0.11.5)