FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28175736
Sequences flagged as poor quality0
Sequence length47-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990330.3514832762487553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA814040.288915256730117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG25900.023.191955
GGTAATA75200.021.1957342
TGTGCGT106500.019.8514048
TAATAAT84700.018.6507534
GTAATAA83050.018.5087473
CGACCCG118500.017.9209615
GGGTAAT104150.016.9037151
GTATTAG76000.016.8754041
TCGGTGG500900.016.13405294-95
TTAGAAC72950.016.0790233
TAATGAC97000.015.7981627
CGGTGGT400100.015.63591994-95
CCGTACG47900.015.6011982
CGCGTGT51650.015.5767581
CCGCGCG112250.015.5479579
GTGTGCG138400.015.5150467
AATACGG38900.015.4414275
GTGCGTC146900.015.0358649
GTATTGG137800.014.9395851
ATATTAG118600.014.7244871
Produced by FastQC (version 0.11.5)