FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19661567
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA214540.1091164300383586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA56700.025.694372
GTATTAG77950.024.3483961
CGTATCA29950.022.67888694-95
CGACCCG77500.019.7138375
CGGTGGT327150.018.75666294-95
TCGGTGG428250.017.75373894-95
TAGGGTC92600.017.7251034
CATAGGG103150.016.8751982
ATAGGGT99450.016.8371583
CCATAGG105750.015.8887621
GTATGAA147250.015.2679391
TAGATAG19700.015.1267185
TAATACT55000.014.53430754
CCGCGCG112600.014.450759
GTATCAT48600.014.34638994-95
TATGAAT145850.014.3021882
TTAGAAC52100.014.2538523
GTTAGGT56650.014.1198111
TGTGCGT71750.014.0419698
TAGGTAC57450.013.8321473
Produced by FastQC (version 0.11.5)