FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39545988
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA503180.12723920312725528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTC53000.028.8550551
TATTAGA149350.024.4875262
GTATTAG225500.022.2563841
ATCCGCG122100.019.52927
CCGTACG72500.018.076422
TGTGCGT126650.017.5574828
TCCGCGA136050.016.76178
TCCGTAC77450.016.6892381
TCGGTGG740800.016.61963394-95
GTATGAA323550.016.5653361
TCTCGAC123200.016.4362453
CGGTGGT627850.016.29714494-95
ATATTAG140350.016.2266141
CGTACGC90800.016.152713
TATGAAT314100.016.1321492
TAGGGTC161000.016.1036474
TTAGAAC97600.015.7544433
TACCCCG115300.015.5517585
CTAGCAT128200.015.4999684
TAGATAG48100.015.4427315
Produced by FastQC (version 0.11.5)