FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005406505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25410987
Sequences flagged as poor quality0
Sequence length35-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA690110.2715793762753096No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT558470.21977501306816613No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT506690.19939800055779022No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA296460.11666607046786494No Hit
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT293430.11547367286441884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275360.10836257560558353No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTC262550.10332144910388565No Hit
TCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCT256310.10086581839579864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)