FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15665949
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1054260.6729627423145575No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT806670.5149193323685657No Hit
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC349000.22277616249101795No Hit
CCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGAC290580.18548509254051573No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA286190.18268283651376627No Hit
CTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGG245880.15695187058249713No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC219740.14026599984463117No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG204270.13039107940412675No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG198250.12654835018293498No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA175330.11191789274942743No Hit
GCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT169620.10827304493331365No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT162490.10372177261652006No Hit
GGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGG159290.10167912585442478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT42500.034.182051
CTTGAAA150500.033.54105894-95
CGGGACG113200.033.2321365
GCTTGAA254450.029.75788394-95
TAGCCCC36400.028.469535
TGTGCGT90050.027.4842958
CTCCCGA186150.024.3783451
GTGTGCG102050.024.1115517
GCTCCGG185650.023.8067761
GCCGTCT1061800.023.59891794-95
GCGTAGC48550.023.3921782
TCCCGAG193550.023.0306592
CGTAGCC47900.023.017493
AGGACGC246500.022.6344789
CCGGGAC170300.022.3397854
GTGCGTC116450.022.0258859
GTGGTGT118800.021.0307644
TGGTGTG138250.020.6390515
GTATTAG122350.020.6144561
GTCGGGG166600.020.4506052
Produced by FastQC (version 0.11.5)