FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36103621
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC534260.14797961678137492No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG501970.13903591553877656No Hit
CCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGAC387270.10726624900034265No Hit
CTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGG380530.10539940024298394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA663800.036.1258794-95
TGTGCGT316300.033.1372038
CGCGTGT128600.033.0202561
CGTGTGG327350.031.3383061
GTGTGGT339500.030.7046892
GTGTGCG352300.030.031257
CGGGACG213600.029.5861135
GTGGTGT373600.028.5091044
TGGTGTG397050.028.0891115
GGTGTGC387950.027.7840636
ACTACTC148250.026.6624263
AGGACGC339850.026.4116219
GTGCGTC409400.026.3651989
ACACCGT94300.026.3043826
CTACTCT159500.025.55354
GCCGTCT3212850.023.92406594-95
GTGTTAT120550.023.9154781
TGTTATG119350.023.6798572
TGTGGTG456050.023.3652783
CTCCCGA296700.023.2950841
Produced by FastQC (version 0.11.5)