FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36103621
Sequences flagged as poor quality0
Sequence length87-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC511390.141645072110634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG473830.13124168348654003No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG415750.11515465443203052No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA372830.1032666501789391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT114000.032.9723131
GTATCAT193000.032.62712594-95
TGTGCGT288300.030.7176178
CGGGACG198400.029.8530675
ACTACTC158250.028.9671923
GTGTGCG325550.027.4789527
CGTGTGG315400.027.3476771
CGGTTCG80250.026.5862964
CTACTCT179350.026.218714
GGTGTGC364550.025.2269926
GTGGTGT360450.025.1333314
GTGCGTC373900.024.5328889
GTGTGGT356550.024.4929222
TACTCTG212450.023.9597285
AGGACGC262950.023.7300119
TGGTGTG412250.023.0654375
ACACCGT89850.023.0085726
CGGTGGG719600.022.72652494-95
GTGTTAT133500.022.4823531
TGTTATG130200.022.0913332
Produced by FastQC (version 0.11.5)