FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17578915
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT393800.023.72896294-95
CGCGTGT54800.022.9665831
GTATTAG49650.022.0134471
TGTGCGT90300.021.8507888
TAGCCCC41500.019.9514375
TAGATAG15600.019.4106275
TGCCGTC532650.018.47404794-95
GCGTAGC47700.018.251342
TATTAGA38750.017.7048242
GTGCGTC115700.017.6266149
GTGTGCG119650.016.7268097
CGACCCG68500.016.6460295
CCGTCTT333350.016.55615694-95
GTATTGG80450.016.4086741
AGGACGC116300.016.3557829
GTGTATA18550.015.8140491
TTAGAAC34000.015.724753
TAGGGTC51550.015.6028684
GTGCGTT38250.015.15265932-33
GGGTATA34900.014.9129161
Produced by FastQC (version 0.11.5)