FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20548616
Sequences flagged as poor quality0
Sequence length88-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA568170.27650037355313856No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT451430.21968876152048392No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT233070.11342369724559552No Hit
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC229290.11158415729799029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT105250.029.0027521
CGGGACG98350.027.9564635
TAGCCCC56100.027.668875
TGTGCGT184650.026.8097938
TAGATAG16850.024.7151475
GCCGTCT355500.024.52333894-95
GTGTGCG206200.024.4882837
TAGGCCC26300.023.5719784
GTGCGTC218100.023.4361679
GTGTGGT212250.023.367262
CGTGTGG217200.022.747951
GTGGTGT228600.022.3991474
GTATTAG42250.022.2909181
GGTGTGC232750.021.9794856
TGGTGTG251750.021.4484845
AATTTAA111550.021.255062
GCGTAGC79300.020.8279442
AGGACGC144450.020.5104549
ATTTAAG115500.020.4865343
ACACCGT58600.020.270186
Produced by FastQC (version 0.11.5)