FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20548616
Sequences flagged as poor quality0
Sequence length58-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA521270.2536764519809996No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT421410.20507950511119583No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT276280.13445187744031034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245490.11946789993058414No Hit
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC224030.10902437419629622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT68400.038.1608281
CGGGACG94750.029.0847475
TGTGCGT151550.026.9635058
TAGCCCC50900.023.6285695
GTGTGCG175700.023.2304157
TAGATAG17400.022.3144445
GTGCGTC200450.021.6376919
CGTATCA17050.021.3588394-95
GTATTAG41300.020.9905281
GGTGTGC211650.020.1570996
ATTTAAG103700.020.1363753
AATTTAA102750.019.9082
ACACCGT54500.019.5482336
AGGACGC132300.019.5413389
AAGCATA110050.018.9314617
TAAGCAT111750.018.516356
GTGGTGT230600.018.3363824
ATATTAG137950.018.1315331
TGGTGTG254750.017.954965
GAGTAGG77950.017.8670771
Produced by FastQC (version 0.11.5)