FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19800096
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA242990.12272162720827214No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT204970.10351970010650452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG20050.023.12555
CGCGTGT41500.021.1034871
GTATTAG54350.020.8175181
TAGCCCC46250.020.664225
GCGTAGC49500.018.6384542
CGACCCG79550.018.4374525
TGTGCGT80400.018.2425278
TATTAGA47650.016.3833052
CTATAGT19350.016.1377284
CTACACT68350.015.436394
TAATACT51100.015.3697434
GTGCGTT43550.014.99237232-33
GTCCTAT54350.014.3719271
TATAGTG23900.014.2532525
CGTAGCC84550.014.21344953
CGGGACG60300.014.201715
GAGTAGG54800.013.9947461
ACACCGT52900.013.9523816
GTGCGTC114050.013.9387369
TTAGAAC42000.013.8559153
Produced by FastQC (version 0.11.5)