FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37870608
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA942780.24894767995274858No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA710920.18772341864698872No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG477680.12613475864976872No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC442210.11676865605115186No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG389900.10295583318863008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTA20450.031.9239852
GTGTTAT383550.025.0260961
TGTTATG389750.024.409412
TATTAGA195950.022.8390052
TTAGACG47600.022.2616564
GTTATGC432450.021.9000233
GTATTAG258950.021.8681131
CCTAATA44800.021.8586672
TTATGCC448700.021.10694
GCCTAAT51000.020.8711031
TACACTC81350.020.2366265
TATGCCC488250.019.678145
TAGGGTC217200.019.0792244
CATAGGG228950.018.6792352
ATGCCCG510200.018.5719496
TAATACA62750.017.9423644
TATGAAT401050.017.7529012
GTATGAA419300.017.6910761
CTTAATA77800.017.6333484
ATAGGGT242650.017.1561093
Produced by FastQC (version 0.11.5)