FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26389010
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA551330.20892409377994856No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG407550.1544392912049372No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG407180.15429908132211098No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC369590.14005451511822536No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA335240.1270377327531423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT715400.022.84751194-95
GTATTAG193950.022.5841851
TATTAGA169950.021.9044252
TGTGCGT107250.021.5680268
TAGCCCC65600.021.3429185
CGACCCG103500.020.4740415
CGCGTGT54550.020.4644681
GCGTAGC71900.019.6707342
TGCCGTC875250.018.84767294-95
TAGGGTC218350.018.6515924
GTGTGCG130150.018.0262037
GCTTGAA230550.018.00864494-95
CATAGGG238700.017.6168252
CCATAGG240600.017.3599431
TAGGCCC41550.017.0760024
GTATGAA340150.016.8683871
CCGCGCG123200.016.7793589
CCGTCTT595950.016.65540994-95
TAATACT142750.016.5344734
TATGAAT335000.016.3791542
Produced by FastQC (version 0.11.5)