FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26422451
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA308700.11683246190900307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269680.10206471761457708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG104000.022.4265845
GTATTAG140350.021.8246881
TGTGCGT103200.021.4543698
TATTAGA130100.020.9820582
ACCCGCG139400.019.3446857
CCGCGCG125800.018.5026539
GTGTGCG122600.018.2909987
TAATACT115150.017.8720684
CGCGTGT51450.017.6673761
CGGGACG88100.017.6135735
TAGGGTC138950.016.5813524
CATAGGG149900.016.4747332
GTGCGTC144600.016.0316189
CGGTTCG31350.015.845339
CTAATAC121050.015.5158393
TCTAATA121550.015.4909362
GACCCGC226850.015.474436
GCGTAGC64500.015.3297872
GTCTAAG56050.015.2882991
ATAGGGT155000.015.0780363
Produced by FastQC (version 0.11.5)