FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9398761
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA241440.2568849234489525No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT198290.21097461676065601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100460.10688642896654144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA24500.029.95346394-95
GTATTAG22100.025.0599021
GTATCAT30750.023.5594194-95
TATTAGA17750.022.9282192
CGGTGGT279600.020.34512794-95
TACACCG27400.018.9980955
TCGGTGG375350.018.54970494-95
CGCGTGT17650.018.2368561
CTAGACT7800.018.2009724
TTAGCCG18350.017.5364134
ACACCGT33300.017.1952726
ACCGTTC19000.016.438358
TGTGCGT37200.015.5197538
GTACTAA27250.015.28628351
CGTTATT26250.015.143292
TTACACC41750.014.8485064
GTGCGTC39950.014.8067989
TACTAAC21250.014.6978982
TCTAGAC18050.014.6817813
GTGTGCG40150.014.6151777
Produced by FastQC (version 0.11.5)