FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005406526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56685719
Sequences flagged as poor quality0
Sequence length87-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT1493400.2634525990576216No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC971270.17134297970181872No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT897620.15835028925010194No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC869410.15337372716397935No Hit
GATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT838220.14787145947641592No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG756760.13350099696186266No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCA697860.12311037282600228No Hit
CATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGC651190.11487725859135701No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA644450.11368824659346739No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG641130.11310256115830515No Hit
GTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCG617440.10892337803812632No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT589380.1039732776433514No Hit
GTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAG584240.10306652368650383No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCA578910.10212625158728252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)