FastQCFastQC Report
Thu 12 May 2022
EGAF00005406527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56685719
Sequences flagged as poor quality0
Sequence length35-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT1400380.2470428221965395No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC894960.15788103525686956No Hit
GATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT862440.15214414057268993No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT860700.1518371849530567No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC836770.14761566312672159No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG706250.1245904634287165No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCA686390.12108693549428208No Hit
CATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGC632810.11163481934488649No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA632530.11158542418770413No Hit
GTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCG625320.11031349889025842No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT610780.1077484789422888No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT606780.10704283383968369No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG601380.10609021295116677No Hit
GTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAG596410.1052134489111799No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580290.10236969914768126No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCA579680.102262088269534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)