FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5933648
Sequences flagged as poor quality0
Sequence length89-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA568580.9582300803822539No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT456370.7691221319498561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT20650.027.9606061
TAGGCCC7550.023.819094
TAGCCCC14600.023.3382425
TGTGCGT40000.023.0727718
TAGATAG9850.023.0618175
AGGACGC53550.021.7424289
GCCGTCT305700.020.64249894-95
GTGTGCG45700.020.4003937
GCGTAGC19500.020.3869132
GCTTGAA91250.020.09034394-95
CGTAGCC20200.019.9138893
CGTGTGG47300.019.7111471
TACACTA8900.019.6743435
CTTGAAA66700.019.03387594-95
TGGTGTG59550.018.357725
CGGGACG19950.018.2657015
GTATTAG9400.018.1251451
GTGCGTC55850.017.8809979
GGTGTGC56250.017.4155166
GTGTGGT54450.017.2097322
Produced by FastQC (version 0.11.5)