FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5933648
Sequences flagged as poor quality0
Sequence length88-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA544910.9183389375305041No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT444190.7485951306852041No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62530.10538205164849686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT19150.032.652951
CGGGACG16350.025.4813395
TAGATAG10300.025.2803345
TGTGCGT36150.022.6566228
GTATTAG10400.022.319271
TAGCCCC15600.021.547215
GTGTGCG39800.020.9356737
GCGTAGC17300.020.5244942
AGGACGC42950.020.3922849
ATACGTA1507.899983E-418.937276
TACACTA8600.018.7170685
CGTAGCC19850.018.603363
TTAGAAC12100.017.6069643
TTAGCGT1901.9151467E-417.442224
GTATTGG22400.017.3413561
CTACACT26550.017.2967514
CCTACAC21650.017.2753373
GCGTGTG38350.016.9126832
GTCCTAG6500.016.7622261
GTGCGTC51800.016.7254819
Produced by FastQC (version 0.11.5)