FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15648496
Sequences flagged as poor quality0
Sequence length89-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA852420.5447296660330807No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT694620.443889304122262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG72100.021.5935541
TGTGCGT90400.020.6778818
TAGCCCC36750.020.0870825
TATTAGA69450.019.0787222
GTGCGTC108200.018.1948139
GCCGTCT127500.018.03204594-95
GTGTGCG107100.017.8943757
AGGACGC101750.017.348269
CGCGTGT66450.016.877841
TAATACT55200.016.1164114
TAGATAG21200.016.0711235
TAGGGTC75500.016.0451074
CTACACT52300.015.924314
GTATTGG66400.015.4651851
GTATGAA111750.015.4564161
CGGGACG53400.015.3304345
TTCCGTC94350.015.109138594-95
CATAGGG84500.015.0086472
GCGTAGC48500.014.6356842
GGGTATA34950.014.6231211
Produced by FastQC (version 0.11.5)