FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15648496
Sequences flagged as poor quality0
Sequence length89-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA846900.5412021704833487No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT645480.41248692526106023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172430.11018950319570647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA29400.025.7525794-95
GTATTAG70400.024.6787431
TATTAGA66150.022.1726282
TGTGCGT67500.021.7290368
CGCGTGT34950.020.5885641
TAGCCCC34950.019.0877385
CGGTGGT312600.018.69938794-95
GTATCAT38600.018.5326394-95
TCGGTGG428200.017.94419194-95
GTGTGCG84850.017.7320067
GTGCGTC94250.016.4655049
TAGGGTC78650.016.2423024
CTTACGT11100.016.1974242
GTTTAGG61850.015.7672941
GTATGAA109600.015.6792661
GTATTGG66300.015.4944371
CGGTGGG170250.015.40108894-95
CATAGGG90150.015.2201032
TAATACT59150.015.1178364
TATGAAT107100.015.0643442
Produced by FastQC (version 0.11.5)