FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23552691
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG24650.025.6852445
TAGCCCC60250.024.5461355
GCCGTCT520350.023.67540494-95
CGCGTGT53500.022.6984581
GCGTAGC63750.022.5324122
GTATTAG65650.020.6568551
TGTGCGT97150.019.3580638
TGCCGTC670600.018.85372294-95
CGACCCG102100.018.2333435
CCGCGCG95850.017.3038279
TAGGCCC40750.017.16054
CGTAGCC97150.016.6333273
TATTAGA50850.016.633222
CCGTCTT442900.016.44339694-95
GTGCGTT59050.015.28299232-33
GCTTGAA176700.014.98549594-95
ATATTAG99300.014.8464411
CGGGACG77050.014.7788355
ATGCCGT688950.014.76503492-93
GTGTGCG130400.014.7111957
Produced by FastQC (version 0.11.5)