FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23552691
Sequences flagged as poor quality0
Sequence length64-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG25050.025.1064535
GTATTAG65350.023.6063021
TAGCCCC58150.022.1187535
CGCGTGT52800.020.9719051
CGACCCG105400.020.592715
GCGTAGC65800.019.331892
TATTAGA52650.019.3102552
CGGTGGT384050.019.20154894-95
TGTGCGT94850.018.94478
TCGGTGG510000.018.44478694-95
CCGCGCG110500.016.603939
GTATCAT61850.016.28872194-95
CGTATCA37150.015.84548494-95
CGTAGCC96350.015.7050643
GTGCGTT52400.015.47652232-33
GTGTGCG125400.015.3852337
CGGGACG74900.015.2151655
CGGTTCG29900.015.1824389
TTAGAAC48100.014.9430743
GTATTGG102650.014.8440671
Produced by FastQC (version 0.11.5)