FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24536640
Sequences flagged as poor quality0
Sequence length85-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA316970.12918231673122318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT667250.023.51023594-95
CGCGTGT48100.022.4326571
GTATTAG124950.022.156981
TATTAGA108500.021.547072
ACCGGTA28750.019.9183988
TGCCGTC809400.019.454994-95
TAGGGTC139800.018.0085094
CATAGGG145950.017.9313092
CCGTCTT531500.017.68335294-95
TGTGCGT96400.017.4775228
TACACCG70250.017.4472395
ACCGTTC48350.017.2275338
GTATGAA199550.016.6722621
TATGAAT192000.016.4898172
ATTAGAG147550.016.3570233
GTGCGTC108550.016.3498469
TAGGAGA107750.015.723134
CCATAGG166100.015.642051
ATAGGGT165950.015.5414843
TAATACT89250.015.5357674
Produced by FastQC (version 0.11.5)