FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24536640
Sequences flagged as poor quality0
Sequence length86-101
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG786727132.06335912333555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG274630.11192649034260599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG252480.10289917445909463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG246320.10038864326982015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC61650.017.9268824
CATAGGG55050.017.7498052
ATAGGGT57750.016.673473
GTATTAG35050.016.3850731
CCATAGG53250.014.8849681
TATTAGA29300.014.0843012
TAATACT27500.013.6262494
GTGTTAC37350.013.5970351
ATTAGAG39200.013.06831553
GTCTTAA14350.012.8992291
TTAGAGG49200.012.5331484
GTCTAAT12900.012.5095081
TACACCG36400.012.2492195
CTATACC16800.012.1406264
TCTATAC13800.012.0301463
CTAGCAT29850.011.6000664
TTACACC35400.010.8533474
ACCGTTC23250.010.8127178
TAGAGGC58800.010.7289265
TAGGACC44100.010.6482564
Produced by FastQC (version 0.11.5)