FastQCFastQC Report
Thu 12 May 2022
EGAF00005406542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57659913
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1158840.20097845100806866No Hit
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT967110.16772657981637953No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG913050.15835091530575152No Hit
TCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTC912040.15817575028252295No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGC696280.12075633898372341No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT685640.11891103616476148No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA666190.11553780873724176No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT657930.11410527102252131No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA620300.10757907317688808No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACG598750.10384164124562587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)