FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005406543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57659913
Sequences flagged as poor quality0
Sequence length35-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1151010.19962048850125738No Hit
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT993320.17227219888451792No Hit
TCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTC926810.16073732195884513No Hit
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG863790.14980771823224914No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT689940.11965678824385323No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA660730.11459087702751129No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGC659360.11435327694649833No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG653830.11339420508664312No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT638250.110692154530306No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA633030.10978684619243181No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT621480.10778372142184814No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT602230.104445180137542No Hit
CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA601240.10427348372863483No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCA601230.10427174942147414No Hit
CCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAA592740.10279932264205809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)