FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12111203
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC31000.028.5343285
TAGATAG13000.027.6540035
GCCGTCT331700.024.35190294-95
GCGTAGC38800.022.7988952
TGTGCGT46650.020.6869648
TGCCGTC421650.020.23766794-95
CGCGTGT30150.018.357031
GTGCGTT31450.018.04880132-33
CGTAGCC51450.017.7443313
TTAGAAC36200.017.11793
GTGTGCG59600.016.6671287
GTATTAG37200.016.5311971
CCGTCTT289550.016.37923694-95
GTATTGG62250.015.6542311
GAGTAGG39600.015.6487621
TAGAACT47950.015.5867734
TACGTAC6850.015.1932388
CGACCCG64050.015.1398815
TAAGGTA28850.015.0844595
ATGCCGT445450.015.01231892-93
Produced by FastQC (version 0.11.5)