FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3970649
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA812072.0451820344734575No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT656241.6527272997436944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT10400.039.538111
CTTGAAA36100.026.60285894-95
GCCGTCT221500.025.01563394-95
GTCCTAG19400.024.8501191
TGTGCGT28150.022.8347368
CGGGACG14800.022.6724955
GTGTGCG29350.022.5435667
GCTTGAA56600.022.4654194-95
TACACTA8250.022.341555
TATGTGT9750.021.3280284
GTGCGTC30400.021.1451999
CCGTCTT170100.020.89398494-95
TGCCGTC259750.020.78552894-95
TCTACGC7750.020.124033
GTATTAG10200.018.9982551
GTGTATG14350.018.7738341
GGTGTGC35400.018.690786
TGGTGTG39150.018.3490875
GTACTAG7800.018.1784421
TGTATGT13050.018.1085642
Produced by FastQC (version 0.11.5)