FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3970649
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA749971.888784427936088No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT591821.4904868196609673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41580.10471839742067354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT9050.035.5515371
TACACTA6700.023.996955
GTCCTAG21550.021.2972011
TGTGCGT22300.020.5692658
ACGTTAA1150.003822598620.5600452
TAATACT8550.019.9107824
GTGCGTC25750.018.73169
CGGTGGT63150.018.6570894-95
TAGGACA8650.018.5872334
TAAGCAT10550.018.3773676
TCGGTGG73700.018.3706394-95
GTGTGCG24250.018.3302357
ATTTAAG10650.018.2048113
ATTCAGT17550.018.0530098
GTGGATT18900.018.0145174
ACACTAT10050.017.880086
CGGGACG15650.017.8274635
AAGCATA10950.017.7060477
GTATTAG11500.017.6916641
CATATTA10350.017.3618162
Produced by FastQC (version 0.11.5)