FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34451927
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC90300.026.2402555
GCCGTCT671700.023.82290394-95
GCGTAGC100100.023.7190782
TGTGCGT175950.023.5751728
CGCGTGT98900.021.7592491
GTATTAG122000.021.2834151
GTGTGCG220350.019.5319867
CGACCCG154100.019.4582925
TGCCGTC916300.019.1182294-95
TATTAGA107050.018.2470042
GTGCGTC239100.018.2192699
TAGATAG45650.018.1307335
CGTAGCC144150.018.1110043
CGGGACG108850.017.9448455
TAGGGTC144500.017.4125294
AGGACGC207150.017.07929
GGCGTAG162450.016.8572851
CCGTCTT603600.016.78314694-95
CGTGTGG247850.016.7355291
GTGCGTT87100.016.64296532-33
Produced by FastQC (version 0.11.5)