FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25143314
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA369810.14708085020136963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA85850.025.951972
CGCGTGT43000.024.3116971
GCCGTCT795650.023.4233494-95
GTATTAG118850.022.4476741
GCTTGAA212600.022.27788494-95
TGTGCGT93000.020.7534078
TGCCGTC936350.020.42368194-95
CCTAATA15550.019.4688032
TAGGGTC122450.018.7739264
CATAGGG130750.018.0891422
ATTAGTA12300.018.0751722
GTGTGCG111200.017.7381387
AATTTAA76850.017.6655732
CCGTCTT630000.017.5100894-95
GAATTTA80300.017.3779261
GCCTAAT20700.017.3673931
GTATGAA223900.017.0072151
ATAGGGT133250.016.9682983
TATGAAT221200.016.8725832
TACCGGT21350.016.8380767
Produced by FastQC (version 0.11.5)