FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25143314
Sequences flagged as poor quality0
Sequence length46-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA359120.14282922290991554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG256630.10206689539811657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA82350.027.2529872
GTATTAG116800.023.2022361
CGCGTGT40200.019.0926251
TAGGGTC126750.018.6028294
ATATTAG74550.018.2394161
CATAGGG137100.017.820162
TGTGCGT88400.017.4607188
AATTTAA69200.017.3790872
ATAGGGT138800.017.1924883
GAATTTA73100.016.9808961
GTTTAGG83550.016.9551411
TTAGTCG35250.016.9242365
GTATTAA12050.016.513491
TTAGTCA82850.016.3444824
CTAGACT16450.016.118324
GTATGAA214900.016.1160131
TATGAAT210650.015.9586542
CCATAGG152600.015.7719671
TATTAGT87700.015.7105992
TAATACT71200.015.6938554
Produced by FastQC (version 0.11.5)