FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19334451
Sequences flagged as poor quality0
Sequence length86-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT272200.021.5372594-95
TAGATAG15750.021.321745
GTATTAG57600.019.3798891
CGACCCG57000.017.0107655
GTGTTAT78050.016.6050191
TGCCGTC361150.016.48268194-95
TATTAGA35000.016.2171232
GTATTGG104450.016.030851
GTTATGC71650.015.9751163
TAGACAG72750.015.4084945
CTATACT26850.015.3256834
TGTTATG79150.014.58140852
CCGTCTT255650.014.53581794-95
TATACTG38050.014.2951285
GGGTATA32250.014.0800171
TAGGTAC31450.013.9864393
ATATTAG48850.013.9431091
TGTGCGT64550.013.8498928
GTGTAGG69000.013.7787141
GTATAAG31600.013.7709051
Produced by FastQC (version 0.11.5)