FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9959166
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123320.12382562957580986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG9850.030.2804585
GTATTAG34800.022.1869531
TTAGAAC21900.021.8341033
TATTAGA21050.021.5913332
CTAGATA15100.019.4389534
CTTAGAA25250.018.56242
TAGGGTC25650.018.2728374
GTATTGG39000.017.9757251
CATAGGG31450.017.763222
TAGAACT34400.017.7537234
ATAGGGT28550.017.245883
ATATTAG31550.017.1157051
TGTGCGT37200.016.9264878
ATTTAAG28900.016.0541153
TAAGGTA23350.016.0176335
ACACCGT26800.015.8988546
TCTAGAT21050.015.7436013
TTAGTCA35000.015.69090754
CCATAGG32650.015.6685891
CCCTTAA14400.015.460541
Produced by FastQC (version 0.11.5)